Fermented Wheat Bran Polysaccharides Improved Intestinal Health of Zebrafish in Terms of Intestinal Motility and Barrier Function

Intestinal barrier dysfunction and gut microbiota disorders have been associated with various intestinal extraintestinal diseases. Fermented wheat bran polysaccharides (FWBP) are promising natural products for enhancing the growth performance antioxidant function of zebrafish. The present study was conducted, in order to investigate effects FWBP on motility zebrafish, which could provide evidence further potential using as a functional food ingredient consideration health. In Experiment 1, normal or loperamide hydrochloride-induced constipation zebrafish larvae were treated three concentrations (10, 20, 40 μg/mL). 2, 180 one month-old healthy randomly divided into groups (six replicates/group 10 zebrafish/tank) fed basal diet, 0.05% FWBP, 0.10% eight weeks. results showed that treatment 6 h can reduce fluorescence intensity alleviate constipation, thereby promoting gastrointestinal When compared control group, diets containing an increased villus height (p < 0.05), up-regulated mRNA expression tight junction protein 1α, muc2.1, muc5.1, matrix metalloproteinases 9 defensin1 abundance phylum Firmicutes decreased Proteobacteria, family Aeromonadaceae, genus Aeromonas 0.05). addition, supplementation IL-10 Occludin1 enhanced Shannon Chao1 indexes Bacteroidota Actinobacteriota at level Additionally, 0.1% significantly improved crypt depth ratio 0.05) IL-17 These findings reveal promote enhance function, thus improving health